Group of Prof. Franz Bracher - Faculty for Chemistry and Pharmacy

Dr. Christoph Müller

Contact

Department of Pharmacy
Center for Drug Research
Butenandtstraße 5-13
D - 81377 Munich

Room: C 2.F7A
Tel. +49-89-2180-77250
Fax. +49-89-2180-77171
eMail. Enable JavaScript to view protected content.

Publications

Schepmann D, Neue C, Westphälinger S, Müller C, Bracher F, Lange C, Bednarski P, Almansa C, Friedland K, Räbiger V, Düfer M, Wünsch B.  Pharmacological characterization of high-affinity sigma-1 receptor ligands with spirocyclic thienopyran and thienofuran scaffold. J. Pharm. Pharmacol., 2020; 72(2):236-248.

Körner A, Zhou E, Müller C, Mohammed Y, Herceg S, Bracher F, Rensen PCN, Wang Y, Mirakaj V, Giera M. Inhibition of Δ24-dehydrocholesterol-reductase activates pro-resolving lipid mediator biosynthesis and inflammation resolution. Proc. Natl. Acad. Sci., 2019; 116(41):20623-20634.

Chiu JE, Thekkiniath J, Mehta S, Müller C, Bracher F, Ben Mamoun C. The yeast pantothenate kinase Cab1 is a master regulator of sterol metabolism and of susceptibility to ergosterol biosynthesis inhibitors. Journal of Biological Chemistry, 2019; 294:14757-14767.

Müller C, Junker J, Bracher F, Giera M. A gas chromatography – mass spectrometry based whole cell screening assay for target identification in distal cholesterol biosynthesis. Nature Protocols, 2019; 14:2546-2570.

Junker J, Chong I, Kamp F, Steiner H, Giera M, Müller C, Bracher F. Comparison of strategies for the determination of steroid sulfates via GC-MS leading to a novel deconjugation derivatization protocol. Molecules, 2019; 24:2353-2570.

Müller C, Wagner AL, Rockinger U, Winter G, Bracher F. Development of a convenient method for the determination of dimethyl sulfoxide in lyophilised pharmaceuticals by static headspace gas chromatography – mass spectrometry. Analytical Methods, 2019; 11:2119-2122.

Müller C, Neugebauer T, Zill P, Lass-Flörl C, Bracher F, Binder U. Sterol composition of clincally relevant mucorales and changes resulting from posaconazole treatment. Molecules, 2018; 23:1218-1230.

van der Vlugt L, Eger K, Müller C, Ninaber DK, Zarcone MC, Amatngalim GD, Bracher F, von Mutius E, Smits HH, Hiemstra PS. Farm dust reduces viral load in human bronchial epithelial cells by increasing barrier function. Journal of Allergy and Clinical Immunology, 2018; 141:1949-1952.

Mailänder-Sánchez D, Braunsdorf C, Grumaz C, Müller C, Lorenz S, Stevens P, Wagener J, Hebecker B, Hube B, Bracher F, Sohn K, Schaller M. Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. PLoS ONE, 2017; 12:e0184438.

Misslinger M, Gsaller F, Müller C, Bracher F, Bromley M, Hass H. The cytochrome b5CybE is regulated by iron availability and is crucial for azole resistance of A. fumigatus. Metallomics, 2017; 9:1655-1665.

Müller C, Hemmers S, Bartl N, Plodek A, Körner A, Mirakaj V, Giera M, Bracher F. A new class of selective and potent inhibitors of human delta 24-dehydrocholesterol reductase. European Journal of Medicinal Chemistry, 2017; 140:305-320.

Fanter L, Müller C, Schepmann D, Bracher F, Wünsch B. Chiral-pool synthesis of 1,2,4-trisubstituted 1,4-diazepanes as novel s1 receptor ligands. Bioorganic and Medicinal Chemistry, 2017; 25:4778-4799.

Vetter F, Müller C, Stöckelhuber M, Bracher F. Determination of coumarin in seasonal bakery products using QuEChERS and GC-MS. Die Pharmazie, 2017; 6:313-316.

Stöckelhuber M, Müller C, Vetter F, Mingo V, Lötters S, Wagner N, Bracher F. Determination of pesticides adsorbed on arthropods and gastropods by a micro-QuEChERS approach and GC-MS/MS. Chromatographia, 2017; 80:825-829.

Müller C, Binder U, Bracher F, Giera M. Antifungal drug testing: Combining minimal inhibitory concentration testing with target identification by the use of gas chromatography mass spectrometry. Nature Protocols, 2017; 12:947-963.

Gsaller F, Hortschansky P, Furukawa T, Carr PD, Rash B, Capilla J, Müller C, Bracher F, Bowyer P, Haas H, Brakhage AA, Bromley MJ. Sterol biosynthesis and azole tolerance is governed by the opposing actions of SrbA and the CCAAT binding complex. PLoS pathogens, 2016; 12:1-22.

Funke F, Matilainen J, Nalenz H, Bechtold-Peters K, Mahler H-C, Vetter F, Müller C, Bracher F, Friess W. Optimization of the bake-on siliconization of cartridges. Part II: Investigations into burn-in time and temperature. European Journal of Pharmaceutics and Biopharmaceutics, 2016; 105:209-222.

Merk H, Zhang S, Lehr T, Müller C, Ulrich M, Bibb JA, Adams RH, Bracher F, Zahler S, Vollmar AM, Liebl J. Inhibition of endothelial Cdk5 reduces tumor growth by promoting non-productive angionesis, Oncotarget 2016; 7:6088-6104.

Keller M, Wolfgardt A, Mülller C, Wilcken R, Böckler FM, Oliaro-Bosso S, Ferrante T, Balliano G, Bracher F. Arylpiperidines as a new class of oxidosqualene cyclase inhibitors. European Journal of Medicinal Chemistry, 2016; 109:13-22.

Keller P, Müller C, Engelhardt I, Hiller E, Lemuth K, Eickhoff H, Wiesmüller K-H, Burger-Kentischer A, Bracher F, Rupp S. An antifungal benzimidazole derivate inhibits ergosterol biosynthesis and reveals novel sterols, Antimicrobial Agents and Chemotherapy, 2015; 59:6296-6307.

Müller C, Binder U, Maurer E, Grimm C, Giera M, Bracher F. Fungal sterol C22-desaturase is not an antimycotic target as shown by selective inhibitors and testing on clinical isolates, Steroids, 2015; 101:1-6.

Müller C, Bracher F. Determination by GC-IT/MS of phytosterols in herbal medicinal products (HMP) for the treatment of lower urinary tract symptoms (LUTS) and food products in marketed in Europe, Planta Medica, 2015; 81:613-620.

Krauss J, Hornacek M, Müller C, Staudacher V, Stadler M, Bracher F. Synthesis and antifungal evaluation of novel N-alkyl tetra- and perhydroquinoline derivatives. Scientia Pharmaceutica, 2015; 83:1-14.

Giera M, Müller C, Bracher F. Analysis and experimental inhibition of distal cholesterol biosynthesis, Chromatographia, 2014; 78:343-358.

Grimm C, Holdt LM, Chen C-C, Hassan S, Müller C, Jörs S, Cuny H, Kissing S, Schröder B, Butz E, Northoff B, Castonguay J, Luber CA, Moser M, Lüllmann-Rauch R, Fendel C, Klugbauer N, Griesbeck O, Haas A, Mann M, Bracher F, Teupser D, Saftig P, Biel M, Wahl-Schott C. High susceptibility to fatty liver disease in two-pore channel 2 deficient mice. Nature Communications, 2014; 5:4699-4712.

Kloos D-P, Gay E, Lingeman H, Bracher F, Müller C, Mayboroda OA, Deelder AM, Niessen WMA, Giera M. Comprehensive gas chromatography-electron ionisation mass spectrometric analysis of fatty acids and sterols using sequential one-pot silylation: quantification and isotopologue analysis. Rapid Communication in Mass Spectrometry, 2014; 28:1507-1514.

Müller C, Vetter F, Richter E, Bracher F. Determination of caffeine, myosmine, and nicotine in chocolate by headspace solid‑phase microextraction coupled with gas chromatography‑tandem mass spectrometry. Journal of Food Science 2014; 79:251-55.

Krauss J, Müller C, Kießling J, Richter S, Staudacher V, Bracher F. Synthesis and biological evaluation of novel N-alkyl tetra- and decahydroisoqinolines: Novel antifungals that target ergosterol biosynthesis. Archiv der Pharmazie 2013; 347:1‑8.

Krojer M, Müller C, Bracher F. Steroidomimetic aminoalkyl spiroacetals as novel inhibitors of the enzyme Δ8/7-sterol isomerase in cholesterol biosynthesis. Archiv der Pharmazie 2013; 346:1‑15.

Krauss J, Gratzl C, Sturm V, Müller C, Staudacher V, Schmidt CQ, Bracher F. Synthesis and biological evaluation of novel alkyl imidazolyl carbionols and their esters: potent antimycotics. Scientia Pharmaceutica 2013; 81:641‑50.

Lange S, Keller M, Müller C, Oliaro‑Bosso S, Balliano G, Bracher F. Aminoproylindenes derived from Grundmann’s ketone as a novel chemotype oxidosqualene cyclase inhibitors. European Journal of Medicinal Chemistry 2013; 63:758‑64.

Müller C, Staudacher V, Krauss J, Giera M, Bracher F. A convenient cellular assay for target identification and selective potency determination of quantification of ergosterol biosynthesis. Steroids 2013; 78:483‑493.

König M, Müller C, Bracher F. Stereoselective synthesis of a new class of potent and selective inhibitors of human Δ8/7-sterol isomerase, Bioorganic and Medicinal Chemistry 2013; 21:1925‑43.

Horling A, Müller C, Barthel R, Bracher F, Imming P. A new class of selective and potent 7-dehydrocholesterol reductase inhibitors. Journal of Medicinal Chemistry 2012; 55:7614‑22.

Müller C, Bracher F, Plössl F. Determination of nicotine in dried mushrooms by using a modified QuEChERS approach and a GC-MS-MS. Chromatographia 2011; 73:807-11.

Research

Overview

Gas chromatography-mass spectrometry (GC-MS), head space (HS) analysis, solid phase micro extraction (SPME), sample preparation (e.g. LLE, QuEChERS).

Ergosterol biosynthesis project

Mechanisms of antifungal drug resistance; Identification of the mode of action of novel antifungals.

Cholesterol biosynthesis project

Identification of novel cholesterol biosynthesis inhibitors; analysis of sterols in body tissues/fluids.

Environmental project

Method development for trace analysis of pesticides from different matrices by GC-MS/MS.

Barn dust project

Preparation of stable barn dust extracts for the prevention and treatment of asthma and allergies.

External Cooperations

Ergosterol biosynthesis project:

Dr. Ulrike Binder Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Innsbruck, Austria.

Dr. Fabio Gsaller Division of Molecular Biology, Medical University of Innsbruck, Innsbruck, Austria.

Associate Prof. Michela Lackner Division of Hygiene and Medical Microbiology, Medical University Innsbruck, Innsbruck, Austria

Associate Prof. Chourki Ben Mamoun Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, USA.

PD Dr. Johannes Wagener Institute for Hygiene and Microbiology, Julius-Maximilians-Universität Würzburg, Würzburg Germany.
 

Cholesterol biosynthesis project:

Dr. Martin Giera Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.

Dr. Frits Kamp Biomedical Center, Metabolic Biochemistry, Ludwig-Maximilians–University Munich

Prof. Valbona Mirakaj Department of Anesthesiology and Intensive Care Medicine, University Hospital Tübingen, Eberhard-Karls University Tübingen, Tübingen, Germany.

Prof. Patrick Renson Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands.

Prof. Harald Steiner Biomedical Center, Metabolic Biochemistry, Ludwig-Maximilians–University Munich

PD Dr. Ivan Tancevski Department of Internal Medicine VI (Infectious Disease), Medical University Innsbruck, Innsbruck, Austria.
 

Environmental project:

Dr. Andreas Kiefer Department of Biogeography, University of Trier, Trier, Germany.

Dr. Anja Baronetzky-Mercier Wildvogel-Pflegestation Kirchwald eV., Kirchwald, Germany.
 

Barn dust project:

Prof. Hamida Hammad Laboratory of Immunoregulation, VIB Inflammation Research Center, Ghent, Belgium.

Prof. Pieter Hiemstra Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands.

Prof. Erika von Mutius German Research Center for Environmental Health, Institute for Asthma and Allergy Prevention, Helmholtz Zentrum München, Neuherberg, Germany.

Prof. Philippe Schmitt-Kopplin German Research Center for Environmental Health, Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany.

Prof. Donata Vercelli Department of Immunobiology, The University of Arizona College of Medicine, Tuscon, USA.

Dr. Ali Önder Yildirim German Research Center for Environmental Health, Immunopthology of COPD, Helmholtz Zentrum München, Neuherberg, Germany.